Immunomodulation by the Pseudomonas syringae HopZ Type III Effector Family in Arabidopsis

• DOI: 10.1371/journal.pone.0116152
• Publication Date: 2014-12-29T18:58:19Z
• Authors: Jennifer D. Lewis; Mike Wilton; G . Adam Mott; Wenwan Lu; Jana A. Hassan; David S. Guttman; Darrell Desveaux
• Keywords: Pseudomonas syringae

Pseudomonas syringae employs a type III secretion system to inject 20–30 different effector (T3SE) proteins into plant host cells. A major role of T3SEs is suppress immune responses and promote bacterial infection. The YopJ/HopZ acetyltransferases are superfamily found in both animal pathogenic bacteria. In P. syringae, this includes the evolutionarily diverse HopZ1, HopZ2 HopZ3 alleles. To investigate roles HopZ family immunomodulation, we generated dexamethasone-inducible T3SE transgenic lines Arabidopsis for members characterized them suppression phenotypes. We show that all can actively various facets immunity catalytic residue-dependent manner. differentially activation mitogen-activated protein (MAP) kinase cascades or production reactive oxygen species, whereas growth non-virulent syringae. Localization studies four containing predicted myristoylation sites localized vicinity plasma membrane while which lacks site at least partially nuclear localized, suggesting diversification immunosuppressive mechanisms. Overall, demonstrate despite significant evolutionary diversification, Arabidopsis.