The Nucleocapsid Protein of Human Coronavirus NL63

• DOI: 10.1371/journal.pone.0117833
• Publication Date: 2015-02-20T18:45:04Z
• Authors: Kaja Zuwała; Anna Golda; Wojciech Kabala; Michał Burmistrz; Michal Zdzalik; Paulina Nowak; Sylwia Kedracka-Krok; Mirosław Zarebski; Jerzy Dobrucki; Dominik Florek; Sławomir Zeglen; Jacek Wojarski; Jan Potempa; Grzegorz Dubin; Krzysztof Pyrc
• Keywords: Coronavirus; Subfamily

Human coronavirus (HCoV) NL63 was first described in 2004 and is associated with respiratory tract disease of varying severity. At the genetic structural level, HCoV-NL63 similar to other members Coronavirinae subfamily, especially human 229E (HCoV-229E). Detailed analysis, however, reveals several unique features pathogen. The coronaviral nucleocapsid protein abundantly present infected cells. It a multi-domain, multi-functional important for viral replication number cellular processes. aim study characterize protein. Biochemical analyses revealed that shares characteristics homologous proteins encoded genomes, N-terminal domain responsible nucleic acid binding C-terminal involved oligomerization. Surprisingly, analysis subcellular localization N that, differently than from species except SARS-CoV, it not nucleus or transfected Furthermore, no significant alteration cell cycle progression cells expressing observed. This stark contrast results obtained coronaviruses, SARS-CoV.