Prostate Field Cancerization: Deregulated Expression of Macrophage Inhibitory Cytokine 1 (MIC-1) and Platelet Derived Growth Factor A (PDGF-A) in Tumor Adjacent Tissue

• DOI: 10.1371/journal.pone.0119314
• Publication Date: 2015-03-13T17:56:57Z
• Authors: Anna C. Jones; Kresta S. Antillon; Shannon M. Jenkins; Sara N. Janos; Heidi N. Overton; Dor S. Shoshan; Edgar G. Fischer; Kristina A. Trujillo; Marco Bisoffi
• Keywords: Field cancerization; Platelet-derived growth factor

Prostate field cancerization denotes molecular alterations in histologically normal tissues adjacent to tumors. Such include deregulated protein expression, as we have previously shown for the key transcription factor early growth response 1 (EGR-1) and lipogenic enzyme fatty acid synthase (FAS). Here add two secreted factors macrophage inhibitory cytokine (MIC-1) platelet derived A (PDGF-A) growing list of markers prostate cancerization. Expression MIC-1 PDGF-A was measured quantitatively by immunofluorescence comprehensively analyzed using methods signal capture several groupings data generated human cancerous (n = 25), 22), disease-free 6) tissues. total 208 digitized images were analyzed. expression tumor elevated 7.1x 23.4x 1.7x 3.7x compared tissues, respectively (p<0.0001 p 0.08 p<0.01 0.23, respectively). In support cancerization, 7.4x 38.4x 1.4x 2.7x, p<0.05 0.51, Also, similar (0.3x 1.0x; p<0.001 0.98 MIC-1; 0.9x 2.6x; 1.00 PDGF-A). All analyses indicated a high level inter- intra-tissue heterogeneity across all types (mean coefficient variation 86.0%). Our shows that is both tumors structurally intact when specimens, defining These could promote tumorigenesis lead multifocality. Among clinical applications, they also be exploited indicators disease false negative biopsies, identify areas repeat biopsy, information surgical margins.