Mechanisms Involved in the Anti-Inflammatory Action of a Polysulfated Fraction from Gracilaria cornea in Rats

• DOI: 10.1371/journal.pone.0119319
• Publication Date: 2015-03-25T18:04:55Z
• Authors: Chistiane Oliveir...; Ricardo Basto Souza; José Ariévilo Gur...; Edfranck de Sousa...; Ianna Wivianne Fe...; Natássia Albuquer...; Annyta Fernandes ...; Kátia Alves Ribeiro; Hellíada Vasconce...; Karuza Maria Alve...; Rodrigo Maranguap...; Mirna Marques Bez...; Norma Maria Barro...
• Keywords: Carrageenan; Anti-inflammatory

The anti-inflammatory mechanisms of the sulfated polysaccharidic fraction obtained from red marine alga Gracilaria cornea (Gc-FI) were investigated using a paw edema model induced in rats by different inflammatory agents (carrageenan, dextran, serotonin, bradykinin, compound 48/80 or L-arginine). Gc-FI at doses 3, 9 27 mg/kg, subcutaneously - s.c., significantly inhibited rat carrageenan and as confirmed myeloperoxidase Evans’ blue assessments, respectively. (9 s.c.) histamine, L-arginine. Additionally, Cg-induced animals with intact mast cells but did not inhibit that degranulated 48/80, revealing protective role on cell membranes. down-regulated IL-1β, TNF-α COX-2 mRNA protein levels compared those group, based qRT-PCR immunohistochemistry analyses. After inhibition ZnPP IX, specific heme oxygenase-1 (HO-1) inhibitor, effect was observed edema, suggesting is, part, dependent integrity HO-1 pathway. can target combination multiple points involved phenomena.