Changes of Phosphatidylcholine and Fatty Acids in Germ Cells during Testicular Maturation in Three Developmental Male Morphotypes of Macrobrachium rosenbergii Revealed by Imaging Mass Spectrometry

• DOI: 10.1371/journal.pone.0120412
• Publication Date: 2015-03-17T19:56:41Z
• Authors: Tanapan Siangcham; Piyachat Chansela; Takahiro Hayasaka; Noritaka Masaki; Morakot Sroyraya; Jaruwan Poljaroen; Saowaros Suwansa-ard; Attakorn Engsusophon; Peter J. Hanna; Prasert Sobhon; Mitsutoshi Setou
• Keywords: Macrobrachium rosenbergii

Testis maturation, germ cell development and function of sperm, are related to lipid composition. Phosphatidylcholines (PCs) play a key role in the structure testes. As well, increases polyunsaturated fatty acids (PUFA) highly unsaturated (HUFA), especially arachidonic acid (ARA), eicosapentaenoic (EPA), docosahexaenoic (DHA) essential for male fertility. This study is first report show composition distribution PCs total (FAs) three groups seminiferous tubules (STs) classified by cellular associations [i.e., A (STs with mostly early cells), B spermatids), C spermatozoa)], morphotypes Macrobrachium rosenbergii, small (SM), orange claw (OC), blue (BC)]. Thin layer chromatography exhibited levels reaching maxima STs group B. Imaging mass spectrometry showed remarkably high signals corresponding PC (16:0/18:1), (18:0/18:2), (18:2/20:5), (16:0/22:6) Moreover, most were detected developing cells intertubular area, but not at area containing spermatozoa. Finally, gas chromatography-mass indicated that major FAs present testes composed 14:0, 16:0, 17:0, 18:0, 16:1, 18:1, 18:2, 20:1, 20:2, 20:4, 20:5, 22:6. The OC contained greatest amounts these while BC least FAs, there was more EPA (20:5) SM than those BC. increasing indicate they important spermatogenesis spermiogenesis. knowledge will be useful formulating diets PUFA HUFA prawn broodstocks order improve testis development, lead increased fecundity.