Prevalence Study and Genetic Typing of Bovine Viral Diarrhea Virus (BVDV) in Four Bovine Species in China

• DOI: 10.1371/journal.pone.0121718
• Publication Date: 2015-04-07T18:50:50Z
• Authors: Mingliang Deng; Sukun Ji; Wentao Fei; Sohail Raza; Chenfei He; Yingyu Chen; Huanchun Chen; Aizhen Guo
• Keywords: Bovine milk; Cattle Diseases

To determine the nationwide status of persistent BVDV infection in different bovine species China and compare test methods, a total 1379 serum samples from clinical healthy dairy cattle, beef yaks (Bos grunniens), water buffalo (Bubalus bubalis) were collected eight provinces 2010 to 2013. The analyzed using commercial antibody (Ab) antigen (Ag) detection kits, RT-PCR based on 5'-UTR Npro gene sequencing. Results showed that overall positive rates for Ab, Ag 58.09% (801/1379), 1.39% (14/1010), 22.64% (146/645), respectively, while individual varied among regions, species, farms. average Ab-positive yaks, 89.49% (298/333), 63.27% (248/392), 45.38% (236/520), 14.18% (19/134), Ag-positive 0.00% (0/116), 0.77% (3/392), 0.82% (3/368), 5.97% (8/134), nucleic acid-positive detected by 32.06% (42/131), 13.00% (26/200), 28.89% (52/180), 19.40% (26/134), respectively. In addition, products sequenced 124 sequences obtained. Phylogenetic analysis indicated all BVDV-positive BVDV-1 subtyped into either BVDV-1b (33.06%), BVDV-1m (49.19%), or new cluster, designated as BVDV-1u (17.74%). confirmed this novel subtype. conclusion, study revealed prevalence dominant subtypes. high proportion bovines with detectable viral acids sera, even presence Ab levels, serious threat health.