Xenotransplantation of Human Adipose-Derived Stem Cells in Zebrafish Embryos

• DOI: 10.1371/journal.pone.0123264
• Publication Date: 2015-04-07T18:53:01Z
• Authors: Jin Li; Guofang Zeng; Yawei Qi; Xudong Tang; Jingjing Zhang; Zeyong Wu; Jie Liang; Lei Shi; Hongwei Liu; Peihua Zhang
• Keywords: Xenotransplantation

Zebrafish is a widely used animal model with well-characterized background in developmental biology. The fate of human adipose-derived stem cells (ADSCs) after their xenotransplantation into the developing embryos zebrafish unknown. Therefore, ADSCs were firstly isolated, and then transduced lentiviral vector system carrying green fluorescent protein (GFP) reporter gene, followed by detection cell viability expression surface antigens. These GFP-expressing transplanted at 3.3–4.3 hour post-fertilization (hpf). Green signal, proliferation differentiation recipient respectively examined using microscopy immunohistochemical staining. results indicated that did not change levels antigens GFP transduction. Microscopic examination demonstrated signals expressed observed larva fish post-transplantation. positive staining Ki-67 revealed survival larvae transplantation. CD105 was observable xenotransplanted ADSCs, but CD31 undetectable. our indicate only can survive proliferate across-species circumstance, also seem to maintain undifferentiation status short term. This xenograft may provide promising useful technical platform for investigation biology physiology vivo.