The Oral Bacterial Communities of Children with Well-Controlled HIV Infection and without HIV Infection

• DOI: 10.1371/journal.pone.0131615
• Publication Date: 2015-07-06T18:47:33Z
• Authors: Brittany E. Goldberg; Emmanuel F. Mongodin; Cheron E. Jones; Michelle Chung; Claire M. Fraser; Anupama Tate; Steven L. Zeichner
• Keywords: Oral Microbiome; Dental plaque

The oral microbial community (microbiota) plays a critical role in human health and disease. Alterations the microbiota may be associated with disorders such as gingivitis, periodontitis, childhood caries, alveolar osteitis, candidiasis endodontic infections. In immunosuppressed population, spectrum of potential disease is even broader, encompassing candidiasis, necrotizing parotid gland enlargement, Kaposi's sarcoma, warts other diseases. Here, we used 454 pyrosequencing bacterial 16S rRNA genes to examine microbiome saliva, mucosal tooth samples from HIV-positive negative children. Patient demographics clinical characteristics were collected cross-section patients undergoing routine dental care. Multiple specimens different sampling sites mouth for each patient. goal study was observe diversity among individual patients, sample locations, HIV status various characteristics. We found that there significant differences enrolled between locations. analysis complicated by uneven enrollment patient cohorts, only five HIV-negative rapid improvement HIV-infected children time conceived completed. generally good limited number plaque could collected. did not identify well-controlled controls, suggesting essentially harbor similar flora compared without HIV. Nor identified teeth or Additional studies are needed better characterize those poorly-controlled