Decreased Expression of CoREST1 and CoREST2 Together with LSD1 and HDAC1/2 during Neuronal Differentiation

• DOI: 10.1371/journal.pone.0131760
• Publication Date: 2015-06-25T18:38:22Z
• Authors: Julián Esteban Sáez; Andrea Verónica G...; Álvaro Patricio B...; Guillermo Eduardo...; Leopoldo Galdames; Marcela González; María Estela Andrés
• Keywords: HDAC1; Corepressor; Demethylase; Mef2

CoREST (CoREST1, rcor1) transcriptional corepressor together with the histone demethylase LSD1 (KDM1A) and deacetylases HDAC1/2 form LSD1-CoREST-HDAC (LCH) complexes to regulate gene expression. CoREST1 belong a family that also comprises CoREST2 (rcor2) CoREST3 (rcor3). represses expression of neuronal genes during differentiation. However, role paralogs in this process is just starting emerge. Here, we report all CoRESTs partners two models differentiation: Nerve-Growth-Factor (NGF)-induced phenotype PC12 cells, vitro maturation embryonic rat cortical neurons. In both models, concomitant gradual decrease LSD1, HDAC1, HDAC2, CoREST1, CoREST2, but not was observed. As required by study, full-length rcor1 identified using silico analysis available genome. The work complemented RNA-seq databases. showed CoRESTs, including four splicing variants CoREST3, display wide adult tissues. Moreover, databases displays higher than mature brain. Immunofluorescent assays immunoblots brain are present glia Regarding functional partnership, interact variants. conclusion, differentiation accompanied decreased core components LCH complexes, CoREST3. combination differential repressor capacity variable protein levels its different should result finely tuned