The Outwardly Rectifying Current of Layer 5 Neocortical Neurons that was Originally Identified as “Non-Specific Cationic” Is Essentially a Potassium Current

• DOI: 10.1371/journal.pone.0132108
• Publication Date: 2015-07-21T14:14:02Z
• Authors: Omer Revah; Lior Libman; Ilya A. Fleidervish; Michael J. Gutnick
• Keywords: Tetraethylammonium; Uniporter

In whole-cell patch clamp recordings from layer 5 neocortical neurons, blockade of voltage gated sodium and calcium channels leaves a cesium current that is outward rectifying. This was originally identified as "non-specific cationic current", subsequently it hypothesized mediated by TRP channels. order to test this hypothesis, we used fluorescence imaging intracellular indicators, found no evidence suggest associated with influx either these ions the cell body or dendrites. Moreover, still prominent in neurons TRPC1-/- TRPC5-/- mice. The effects on various blocking agents, especially its sensitivity tetraethylammonium, not non-specific current, but rather generated cesium-permeable delayed rectifier potassium