Proteome Analysis of Poplar Seed Vigor

• DOI: 10.1371/journal.pone.0132509
• Publication Date: 2015-07-14T18:02:56Z
• Authors: Hong Zhang; Wei-Qing Wang; Shu-Jun Liu; Ian Max Møller; Song-Quan Song
• Keywords: Proteome; Protein metabolism

Seed vigor is a complex property that determines the seed's potential for rapid uniform emergence and subsequent growth. However, mechanism change in seed poorly understood. The seeds of poplar (Populus × Canadensis Moench), which are short-lived, were stored at 30°C 75±5% relative humidity different periods time (0–90 days) to obtain (from 95 0% germination). With decreasing vigor, temperature range germination became narrower; respiration rate decreased markedly, while electrolyte leakage increased both levelling off after 45 days. A total 81 protein spots showed significant abundance (≥ 1.5-fold, P < 0.05) when comparing proteomes among with vigor. Of identified 65 proteins, most belonged groups involved metabolism (23%), synthesis destination (22%), energy (18%), cell defense rescue (17%), storage (15%). These proteins accounted 95% all proteins. During aging, 53 6 consistently abundance, respectively, they associated (19%), (15%), growth structure (3%). data show decrease (aging) an energy-dependent process, requires degradation as well cellular rescue.