Immunohistochemistry and Fluorescence In Situ Hybridization Can Inform the Differential Diagnosis of Low-Grade Noninvasive Urothelial Carcinoma with an Inverted Growth Pattern and Inverted Urothelial Papilloma

• DOI: 10.1371/journal.pone.0133530
• Publication Date: 2015-07-24T14:05:38Z
• Authors: Juan-Juan Sun; Yong Wu; Yong-Ming Lu; Hui-Zhi Zhang; Tao Wang; Xiao-Qun Yang; Meng-Hong Sun; Chao-Fu Wang
• Keywords: Inverted Papilloma; Tissue microarray; Subtyping; Carcinoma in situ; Comparative genomic hybridization

Urothelial carcinoma (UC) comprises a heterogeneous group of epithelial neoplasms with diverse biological behaviors and variable clinical outcomes. Distinguishing UC histological subtypes has become increasingly important because prognoses therapy can dramatically differ among subtypes. In work, overlapping morphological findings between low-grade noninvasive (LGNUC), which exhibits an inverted growth pattern, urothelial papilloma (IUP) make subclassification difficult. We propose combination immunohistochemistry (IHC) molecular cytogenetics for subtyping these entities. our study, tissue microarray immunohistochemical profiles Ki-67, p53, cytokeratin 20 (CK20) cyclinD1 were assessed. Molecular genetic alterations such as the gain chromosomes 3, 7 or 17 homozygous loss 9p21 also assessed their usefulness in differentiating conditions. Based on analysis, Ki-67 CK20 may be useful differential diagnosis two tumor types. Fluorescence situ hybridization (FISH) provide data cases malignant nature neoplasm is unclear. LGNUC pattern that negative both positively detected using FISH.