Molecular and Cytogenetic Characterization of Wild Musa Species

• DOI: 10.1371/journal.pone.0134096
• Publication Date: 2015-08-07T14:05:28Z
• Authors: Jana Čížková; Eva Hřibová; Pavla Christelová; Ines Van den Houwe; Markku Häkkinen; Nicolas Roux; Rony Swennen; Jaroslav Doležel
• Keywords: Germ plasm; Musa acuminata

The production of bananas is threatened by rapid spreading various diseases and adverse environmental conditions. preservation characterization banana diversity essential for the purposes crop improvement. world's largest germplasm collection maintained at Bioversity International Transit Centre (ITC) in Belgium continuously expanded new accessions edible cultivars wild species. Detailed morphological molecular necessary efficient management utilization diversity. In this work, nuclear DNA content genomic distribution 45S 5S rDNA were examined 21 diploid recently added to ITC collection, representing both sections genus Musa. 2C section Musa ranged from 1.217 1.315 pg. Species belonging Callimusa had contents ranging 1.390 1.772 While number loci was conserved Musa, it highly variable rRNA gene clusters found on two eight chromosomes per cell. genotyped using a set 19 microsatellite markers establish their relationships with remaining held ITC. Genetic done SSR genotyping platform extended phylogenetic analysis ITS region. sequence data supported clustering obtained most accessions. High level nucleotide presence more than types sequences diploids pointed origin hybridization different genotypes. This study significantly expands species where genome size known. identified that are closely related previously characterized provided aid classification. Sequence region further information about evolutionary between individual suggested some analyzed interspecific hybrids and/or backcross progeny.