Characterization of Sin1 Isoforms Reveals an mTOR-Dependent and Independent Function of Sin1γ

• DOI: 10.1371/journal.pone.0135017
• Publication Date: 2015-08-11T17:54:01Z
• Authors: Yuanyang Yuan; Bangfen Pan; Haipeng Sun; Guoqiang Chen; Bing Su; Ying Huang
• Keywords: 0301 basic medicine; Science; TOR Serine-Threonine Kinases; Q; R; Mechanistic Target of Rapamycin Complex 2; Cell Line; Protein Transport; 03 medical and health sciences; Tubulin; Gene Knockdown Techniques; Multiprotein Complexes; Medicine; Humans; Protein Isoforms; Cilia; Research Article; Adaptor Proteins, Signal Transducing; Protein Binding; Signal Transduction

Sin1 or MAPKAP1 is a key component of mTORC2 signaling complex which necessary for AKT phosphorylation at the S473 and T450 sites, also downstream as well. A number splicing variants have been reported that can produce different isoforms due to exon skipping alternative transcription initiation. In this report, we characterized four isoforms, including novel isoform 3' termination 9a, termed Sin1γ. Sin1γ expression be detected in multiple adult mouse tissues, it encodes C-terminal truncated protein comparing full length Sin1β isoform. contrast Sin1β, overexpression deficient embryonic fibroblasts has no significant impact on activity subunits level, although still interact with components. More interestingly, was specific cytosolic location distinct feature structure, its localization transiently disrupted during cell cycle. Therefore, may properties intracellular from other isoforms.