Antimicrobial Susceptibility of Bordetella bronchiseptica Isolates from Swine and Companion Animals and Detection of Resistance Genes

• DOI: 10.1371/journal.pone.0135703
• Publication Date: 2015-08-14T18:13:32Z
• Authors: Sandra Prüller; Ulrike Rensch; Diana Meemken; Heike Kaspar; Peter A. Kopp; Günter Klein; Corinna Kehrenberg
• Keywords: Bordetella bronchiseptica; Broth microdilution; Amp resistance; Florfenicol

Bordetella bronchiseptica causes infections of the respiratory tract in swine and other mammals is a precursor for secondary with Pasteurella multocida. Treatment B. conducted primarily antimicrobial agents. Therefore it essential to get an overview susceptibility status these bacteria. The aim this study was comparatively analyse broth microdilution testing according CLSI recommendations incubation time 16 20 hours longer 24 hours, as recently proposed obtain more homogenous MICs. Susceptibility against panel 22 agents two fixed combinations performed 107 porcine isolates from different farms regions Germany 43 obtained companion animals European countries. Isolates increased MICs were investigated by PCR assays presence resistance genes. For ampicillin, all classified resistant, whereas only single isolate resistant florfenicol. All showed elevated β-lactam antibiotics demonstrated overall low cephalosporines. Extension resulted 1–2 dilution steps higher MIC50 values seven tested, while exhibited twofold MIC50/90 tetracycline cefotaxime. three agents, lower MIC90 detected both, animal isolates. Among 150 genes blaBOR-1 (n = 147), blaOXA-2, 4), strA strB 17), sul1 10), sul2 73), dfrA7 3) tet(A) 8) plasmid localisation identified several