Ultraconserved Elements Sequencing as a Low-Cost Source of Complete Mitochondrial Genomes and Microsatellite Markers in Non-Model Amniotes

• DOI: 10.1371/journal.pone.0138446
• Publication Date: 2015-09-17T18:29:05Z
• Authors: Fábio Raposo do A...; Leandro G. Neves; Márcio F. R. Resende; Flávia Mobili; Cristina Y. Miyaki; Katia C. M. Pelle...; Cibele Biondo
• Keywords: Genetic Markers; 0301 basic medicine; Science; Q; Molecular Sequence Data; R; Sequence Analysis, DNA; DNA, Mitochondrial; 03 medical and health sciences; Genome, Mitochondrial; Medicine; Animals; Finches; Brazil; Phylogeny; Research Article; Microsatellite Repeats

Sequence capture of ultraconserved elements (UCEs) associated with massively parallel sequencing has become a common source of nuclear data for studies of animal systematics and phylogeography. However, mitochondrial and microsatellite variation are still commonly used in various kinds of molecular studies, and probably will complement genomic data in years to come. Here we show that besides providing abundant genomic data, UCE sequencing is an excellent source of both sequences for microsatellite loci design and complete mitochondrial genomes with high sequencing depth. Identification of dozens of microsatellite loci and assembly of complete mitogenomes is exemplified here using three species of Poospiza warbling finches from southern and southeastern Brazil. This strategy opens exciting opportunities to simultaneously analyze genome-wide nuclear datasets and traditionally used mtDNA and microsatellite markers in non-model amniotes at no additional cost.