Orally-Induced Intestinal CD4+ CD25+ FoxP3+ Treg Controlled Undesired Responses towards Oral Antigens and Effectively Dampened Food Allergic Reactions

• DOI: 10.1371/journal.pone.0141116
• Publication Date: 2015-10-30T14:25:50Z
• Authors: Paola Lorena Smal...; María Lucía Orsin...; Carlos Alberto Fo...; Guillermo Horacio...
• Keywords: Allergic response; Lamina propria; Cholera toxin

The induction of peripheral tolerance may constitute a disease-modifying treatment for allergic patients. We studied how oral immunotherapy (OIT) with milk proteins controlled allergy in sensitized mice (cholera toxin plus proteins) upon exposure to the allergen. Symptoms were alleviated, skin test was negativized, serum specific IgE and IgG1 abrogated, substantial reduction secretion IL-5 IL-13 by antigen-stimulated spleen cells observed, while gene expression jejunum down-regulated, IL-10 TGF-β increased. In addition, we observed an CD4+CD25+FoxP3+ IL-10- TGF-β-producing regulatory T lamina propria. Finally, transfer experiments confirmed central role these induction. demonstrated that administration pre- or post-sensitization Th2-immune response through elicitation mucosal Tregs inhibited hypersensitivity symptoms response.