dTAF10- and dTAF10b-Containing Complexes Are Required for Ecdysone-Driven Larval-Pupal Morphogenesis in Drosophila melanogaster

• DOI: 10.1371/journal.pone.0142226
• Publication Date: 2015-11-10T14:04:10Z
• Authors: Zoltan Pahi; Zsuzsanna Kiss; Orbán Komonyi; Barbara N. Borsos; Laszlo Tora; Imre M. Boros; Tibor Pankotai
• Keywords: Polytene chromosome; Ecdysone receptor; RNA polymerase II

In eukaryotes the TFIID complex is required for preinitiation assembly which positions RNA polymerase II around transcription start sites. On other hand, histone acetyltransferase complexes including SAGA and ATAC, modulate at several steps through modification of specific core residues. this study we investigated function Drosophila melanogaster proteins TAF10 TAF10b, are subunits dTFIID dSAGA, respectively. We generated a mutation eliminated production both orthologues. The simultaneous deletion dTaf10 genes impaired recruitment subunit dTAF5 to polytene chromosomes, while binding subunits, dTAF1 RNAPII was not affected. lack dTAF10 resulted in failures larval-pupal transition during metamorphosis transcriptional reprogramming developmental stage. Surprisingly, unlike dSAGA mutations, dATAC mutations very similar changes steady state mRNA levels approximately 5000 as did ablation genes, indicating that dTAF10- and/or dTAF10b-containing affect pathways. Importantly, phenotype resulting from dTaf10+dTaf10b could be rescued by ectopically added ecdysone, suggesting involved expression ecdysone biosynthetic genes. Indeed, mutants, cytochrome regulate synthesis ring gland, were underrepresented. Therefore our data support idea presence only steps. propose distinct forms exist metamorphosis, wherein different TAF compositions serve target stages tissues.