Species Specific Differences of CD1d Oligomer Loading In Vitro

• DOI: 10.1371/journal.pone.0143449
• Publication Date: 2015-11-24T21:34:06Z
• Authors: Daniel Paletta; Alina Suzann Fich...; Lisa Starick; Steven A. Porcelli; Paul B. Savage; Thomas Herrmann
• Keywords: CD1D; Oligomer

CD1d molecules are MHC class I-like that present glycolipids to iNKT cells. The highly conserved interaction between CD1d:α-Galactosylceramide (αGC) complexes and the TCR not only defines this population of αβ T cells but can also be used for its direct identification. Therefore, oligomers a widely tool cell related investigations. To end, lipid chains antigen have inserted into hydrophobic pockets binding cleft, often with help surfactants. In study, we investigated influence different surfactants (Triton X-100, Tween 20, Tyloxapol) on in vitro loading derived from four species (human, mouse, rat cotton rat) αGC derivatives carrying modifications acyl-chain (DB01-1, PBS44) 6-acetamido-6-deoxy-addition at galactosyl head group (PBS57). We compared dimers tetramers staining an transductant was as readout efficacy. results underlined importance efficacy proper analysis demonstrated necessity adjust conditions each oligomer/glycolipid combination. efficient usage revealed species-specific depending origin producing Additional variation surfactant-dependent tested influenced by length modification PBS57 showing least dependence lowest degree species-dependent differences.