Effect of P144® (Anti-TGF-β) in an “In Vivo” Human Hypertrophic Scar Model in Nude Mice

• DOI: 10.1371/journal.pone.0144489
• Publication Date: 2015-12-31T18:38:52Z
• Authors: Shan Shan Qiu; Javier Dotor; Bernardo Hontanilla
• Keywords: 0301 basic medicine; Cicatrix, Hypertrophic; Science; Q; Microfilament Proteins; R; Antibodies, Monoclonal; Mice, Nude; Fibrillins; Immunohistochemistry; 3. Good health; Disease Models, Animal; Mice; 03 medical and health sciences; Transforming Growth Factor beta; Medicine; Animals; Heterografts; Humans; Collagen; Biomarkers; Research Article

Hypertrophic scars are one of the most important complications in surgery due to their cosmetic and functional impairments. Previous studies tissue fibrotic disorders have shown promising results by inhibiting biological activity effect Transforming Growth Factor-beta 1 (TGF-β1). The aim current study was determine clinical inhibition TGF-β1 signaling human hypertrophic implanted nude mice topical application an inhibitor (P144®).A total 30 were 60 mice. animals divided two groups, group A (placebo) B (treatment). Group C (basal) considered as preimplanted scar samples they not After shedding period, a lipogel containing placebo (group A) or P144 B) daily administered during weeks. sacrificed upon completion study. Total area, thickness collagen fibers area measure compared across all groups. Immunohistochemistry also performed order quantify type I III elastic fiber expressions present dermis.Successful achieved 83,3% xenografts. mean time for 35±5.4 days. Statistically significant differences found between Increased decreased P144-treated basal group.Topical may promote maturation improvement morphology features "in vivo" model after weeks treatment.