Genome-Wide Dynamic Profiling of Histone Methylation during Nuclear Transfer-Mediated Porcine Somatic Cell Reprogramming

• DOI: 10.1371/journal.pone.0144897
• Publication Date: 2015-12-18T18:44:28Z
• Authors: Zubing Cao; Yunsheng Li; Zhen Chen; Heng Wang; Meiling Zhang; Naru Zhou; Ronghua Wu; Yinghui Ling; Fugui Fang; Ning Li; Yunhai Zhang
• Keywords: Reprogramming; Inner cell mass; H3K4me3; Histone Methylation

The low full-term developmental efficiency of porcine somatic cell nuclear transfer (SCNT) embryos is mainly attributed to imperfect epigenetic reprogramming in the early embryos. However, dynamic expression patterns histone methylation involved progression during SCNT embryo development remain be unknown. In this study, we characterized and compared multiple markers including transcriptionally repressive (H3K9me2, H3K9me3, H3K27me2, H3K27me3, H4K20me2 H4K20me3) active modifications (H3K4me2, H3K4me3, H3K36me2, H3K36me3, H3K79me2 H3K79me3) from different stages with that vitro fertilization (IVF) counterparts. We found level H3K9me2, H3K9me3 H4K20me3 1-cell 4-cell was significantly higher than IVF also detected a symmetric distribution pattern H3K9me2 between inner mass (ICM) trophectoderm (TE) blastocysts. both lineages expanded blastocyst onwards lower morula stage no aberrant H3K27me2/3 occurred H3K4me3 at H3K4me2 8-cell Dynamic other were similar Taken together, exhibited developmentally stage-specific abnormal