Anthrax Toxin Receptor 1 Is Essential for Arteriogenesis in a Mouse Model of Hindlimb Ischemia

• DOI: 10.1371/journal.pone.0146586
• Publication Date: 2016-01-19T20:41:30Z
• Authors: N. J. Andersen; E. A. Boguslawski; A. S. Naidu; C. Szot; J. L. Bromberg-White; K. Kits; C. Y. Kuk; L. E. Holton; B. St. Croix; C. M. Chambers; N. S. Duesbery
• Keywords: Arteriogenesis; Anthrax toxin

Anthrax toxin receptor 1/tumor endothelial marker 8 (Antxr1 or TEM8) is up-regulated in tumor vasculature and serves as a for anthrax toxin, but its physiologic function unclear. The objective of this study was to evaluate the role Antxr1 arteriogenesis. arteriogenesis tested by measuring gene expression immunohistochemistry mouse model hindlimb ischemia using wild-type ANTXR1-/- mice. Additional tests were performed vitro models fluid shear stress hypoxia, well human muscle tissues obtained from patients having peripheral artery disease. We observed that transiently increased ischemic following femoral ligation necessary In absence Antxr1, mean arterial lumen area decreased. mRNA protein positively regulated stress, not hypoxia. Furthermore, elevated disease requiring lower extremity bypass surgery. These findings demonstrate an essential disease, with important implications managing other arteriogenesis-dependent vascular diseases.