A New Glabrous Gene (csgl3) Identified in Trichome Development in Cucumber (Cucumis sativus L.)

• DOI: 10.1371/journal.pone.0148422
• Publication Date: 2016-02-04T18:37:48Z
• Authors: Jin-Ying Cui; Han Miao; Li-Hong Ding; Todd C. Wehner; Pan-Na Liu; Ye Wang; Sheng-Ping Zhang; Xing-Fang Gu
• Keywords: Bulked segregant analysis; Cucumis; Indel; Trichome; Candidate gene

Spines or trichomes on the fruit of cucumbers enhance their commercial value in China. In addition, glabrous mutants exhibit resistance to aphids and therefore use by growers can reduce pesticide residues. Previous studies have reported two mutant plants containing genes, csgl1 csgl2. present study, a new mutant, NCG157, was identified showing gene interaction effect with This showed character stems, leaves, tendrils, receptacles ovaries, there were no spines tumors surface. Inheritance analysis that single recessive gene, named csgl3, determined trait. An F2 population derived from cross inbred lines 9930 (a fresh market type Northern China exhibits trichomes) NCG157 (an American processing surfaces) used for genetic mapping csgl3 gene. By combining bulked segregant (BAS) molecular markers, 18 including simple sequence repeats (SSR), nine insertion deletions (InDel) seven cleaved amplified polymorphism sequences (dCAPs), link All linked markers as anchor loci locate cucumber chromosome 6. The mapped between dCAPs dCAPs-21 dCAPs-19, at distances 0.05 cM 0.15 cM, respectively. physical distance this region 19.6 kb. Three InDel-19, dCAPs-2 dCAPs-11, co-segregated csgl3. There candidate genes region, Csa6M514860 Csa6M514870. Quantitative real-time PCR expression Csa6M514870 higher tissues than consistent phenotypic characters. is postulated be development cucumber. study will facilitate marker-assisted selection (MAS) smooth plant trait breeding provide future cloning