Intestinal Cell Tight Junctions Limit Invasion of Candida albicans through Active Penetration and Endocytosis in the Early Stages of the Interaction of the Fungus with the Intestinal Barrier

• DOI: 10.1371/journal.pone.0149159
• Publication Date: 2016-03-02T18:46:09Z
• Authors: Marianne Goyer; Alicia Loiselet; Fabienne Bon; Coralie L’Ollivier; Michael Laue; Gudrun Holland; Alain Bonnin; Frederic Dalle
• Keywords: Internalization

C. albicans is a commensal yeast of the mucous membranes in healthy humans that can also cause disseminated candidiasis, mainly originating from digestive tract, vulnerable patients. It necessary to understand cellular and molecular mechanisms interaction with enterocytes better basis commensalism pathogenicity improve management candidiasis. In this study, we investigated kinetics tight junction (TJ) formation parallel invasion into Caco-2 intestinal cell line. Using invasiveness assays on cells displaying pharmacologically altered TJ (i.e. differentiated epithelial treated EGTA or patulin), were able demonstrate protect against albicans. Moreover, treatment pharmacological inhibitor endocytosis decreased fungus TJ, suggesting facilitating access basolateral side promotes its hyphal form. These data supported by SEM observations which highlighted membrane protrusions engulfing hyphae. We furthermore demonstrated Als3, hypha-specific invasin, facilitates internalization active penetration induced TJ. However, our failed binding Als3 E-cadherin as trigger mechanism