Probiotic Gut Microbiota Isolate Interacts with Dendritic Cells via Glycosylated Heterotrimeric Pili

• DOI: 10.1371/journal.pone.0151824
• Publication Date: 2016-03-17T17:51:57Z
• Authors: Hanne L. P. Tytgat; Nienke H. van Tei...; Ruby May A. Sullan; François P. Douil...; Pia Rasinkangas; Marcel Messing; Justus Reunanen; Reetta Satokari; Jos Vanderleyden; Yves F. Dufrêne; Teunis B. H. Geij...; Willem M. de Vos; Sarah Lebeer
• Keywords: Sortase; Lactobacillus rhamnosus; Glycobiology

Mapping of the microbial molecules underlying microbiota-host interactions is key to understand how microbiota preserve mucosal homeostasis. A pivotal family such bacterial are pili. Pili proteinaceous cell wall appendages with a well-documented role in adhesion, whilst their immune interaction host less established. Gram-positive pili often posttranslationally modified by sortase-specific cleavage reactions and formation intramolecular peptide bonds. Here we report glycosylation as new level posttranslational modification sortase-dependent beneficial species its modulation. We focused on SpaCBA model probiotic human gut isolate Lactobacillus rhamnosus GG. unique combination molecular techniques, nanoscale mechanical immunological approaches led identification mannose fucose residues These glycans recognized dendritic cells via C-type lectin receptor DC-SIGN, carbohydrate-dependent tailoring pattern recognition receptor. This specific lectin-sugar moreover functional importance modulated cytokine response cells. provides insight into direct glycoproteins can play immunomodulation host. Modification complex heterotrimeric for DC-SIGN Our findings shed light yet underappreciated glycoconjugates bacteria-host interactions.