Pulchrin A, a New Natural Coumarin Derivative of Enicosanthellum pulchrum, Induces Apoptosis in Ovarian Cancer Cells via Intrinsic Pathway

• DOI: 10.1371/journal.pone.0154023
• Publication Date: 2016-05-02T17:32:57Z
• Authors: Noraziah Nordin; Mehran Fadaeinasab; Syam Mohan; Najihah Mohd Hashim; Rozana Othman; Hamed Karimian; Venus Iman; Noorlela Ramli; Hapipah Mohd Ali; Nazia Abdul Majid
• Keywords: DNA laddering

Drug resistance presents a challenge in chemotherapy and has attracted research interest worldwide particular attention been given to natural compounds overcome this difficulty. Pulchrin A, new compound isolated from products demonstrated novel potential for development as drug. The identification of pulchrin A was conducted using several spectroscopic techniques such nuclear magnetic resonance, liquid chromatography mass spectrometer, infrared ultraviolet spectrometry. cytotoxicity effects on CAOV-3 cells indicates that is more active than cisplatin, which an IC50 22.3 μM. Significant changes cell morphology were present, membrane blebbing formation apoptotic bodies. involvement phosphatidylserine (PS) apoptosis confirmed by Annexin V-FITC after 24 h treatment. Apoptosis activated through the intrinsic pathway activation procaspases 3 9 well cleaved caspases ended at executioner pathway, with occurrence DNA laddering. further via gene protein expression levels, Bcl-2 down-regulated Bax up-regulated. Furthermore, cycle disrupted G0/G1 phase, leading apoptosis. Molecular modeling proteins high- binding affinity, inhibited function led death. Results current study can shed light therapeutic agents, particularly, human ovarian cancer treatments.