Dissecting the Effects of Ischemia and Reperfusion on the Coronary Microcirculation in a Rat Model of Acute Myocardial Infarction

• DOI: 10.1371/journal.pone.0157233
• Publication Date: 2016-07-08T18:25:18Z
• Authors: Maurits R. Hollander; Guus A. de Waard; Lara S. F. Konijn...; Rosalie M. E. Mei...; Charissa E. van d...; Nanne Paauw; Helga E. de Vries; Peter M. van de Ven; Jurjan Aman; Geerten P. Van Ni...; Peter L. Hordijk; Hans W. M. Niessen; Anton J. G. Horre...; Niels Van Royen
• Keywords: Vascular permeability; Extravasation

Microvascular injury (MVI) after coronary ischemia-reperfusion is associated with high morbidity and mortality. Both ischemia reperfusion are involved in MVI, but to what degree these phases contribute unknown. Understanding the etiology essential for development of new potential therapies.Rats were divided into 3 groups receiving either 30 minutes ischemia, 90 or followed by 60 reperfusion. Subsequently hearts ex-vivo perfused a Langendorff-model. Fluorescence electron microscopy was used analysis capillary density, vascular permeability ultrastructure. Most MVI observed In comparison 30' 90' group, wall thickness decreased (207.0±74 vs 407.8±75 407.5±71, p = 0.02). Endothelial nuclei 30'-60' group showed irreversible damage chromatin density variation (50.5±9.4, 35.4±7.1 23.7±3.8, 0.03). Cell junction lowest (0.15±0.02 2.5±0.6 1.8±0.7, p<0.01). Microsphere extravasation increased both group.Ischemia alone induces mild morphological changes microcirculation, permeability. Ischemia minutes, reperfusion, massive MVI. This shows direct consequences on microcirculation. These data imply that therapeutic window exists protect microcirculation directly upon revascularization.