Physiological and Proteomic Investigations to Study the Response of Tomato Graft Unions under Temperature Stress

• DOI: 10.1371/journal.pone.0157439
• Publication Date: 2016-06-16T19:17:27Z
• Authors: Sowbiya Muneer; Chung Ho Ko; Hao Wei; Yuze Chen; Byoung Ryong Jeong
• Keywords: Plant Somatic Embryogenesis Techniques; Proteomics; 0301 basic medicine; Science; Photoperiod; Plant Roots; Antioxidants; 03 medical and health sciences; Ascorbate Peroxidases; Solanum lycopersicum; Gene Expression Regulation, Plant; Stress, Physiological; Photosynthesis; Plant Proteins; 2. Zero hunger; Q; R; Molecular Sequence Annotation; Hydrogen Peroxide; 15. Life on land; Catalase; Adaptation, Physiological; Droughts; Plant Leaves; Gene Ontology; Medicine; Plant Shoots; Research Article; Signal Transduction

Background Grafting is an established practice for asexual propagation in horticultural and agricultural crops. The study on graft unions has become of interest horticulturists using proteomic genomic techniques to observe transfer genetic material signal transduction pathways from root shoot root. Another reason the was potentially resistance against abiotic stresses. Using physiological analyses, we investigated (rootstock scions) tomato genotypes exposed standard-normal (23/23 25/18°C day/night) high-low temperatures (30/15°C day/night). Results Graft had varied responses diverse temperatures. High-low temperature, but not induced production reactive oxygen species (ROS) form H2O2 O2-1 rootstock scions. However, expression many cell protection molecules also induced, including antioxidant enzymes their immunoblots, which show increase activities such as superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX). interfaces thus actively defend stress by modifying establish a new cellular homeostasis. As result, proteins defense were regulated under addition regulation photosynthetic proteins, ion binding/transport protein synthesis. Moreover, biomass, hardness, vascular transport activity evaluated investigate basic connectivity between Conclusions Our provides evidence grafted plants' response temperature. Most notably, our novel insight into mechanisms used adapt temperature (rootstock/scion).