Parathyroid Hormone-Related Peptide (1-36) Enhances Beta Cell Regeneration and Increases Beta Cell Mass in a Mouse Model of Partial Pancreatectomy

• DOI: 10.1371/journal.pone.0158414
• Publication Date: 2016-07-08T14:10:34Z
• Authors: Anaïs Mozar; Hugo Lin; Katoura Williams; Connie Chin; Rosemary Li; Nagesha Guthalu K...; Andrew F. Stewart; Adolfo Garcia-Ocaña; Rupangi Chhaya Va...
• Keywords: Parathyroid hormone-related protein; Homeostasis; BETA (programming language)

Aims/Hypothesis Finding ways to stimulate the regeneration of endogenous pancreatic beta cells is an important goal in treatment diabetes. Parathyroid hormone-related protein (PTHrP), full-length (1–139) and amino-terminal (1–36) peptides, enhance cell function, proliferation, survival. Therefore, we hypothesize that PTHrP(1–36) has potential regenerate cells. Methods The partial pancreatectomy (PPx) mouse model injury was used test this hypothesis. Male Balb/c mice underwent either sham-operation or PPx, were subsequently injected with (160μg/kg) vehicle (veh), for 7, 30, 90 days. four groups mice, sham-veh, sham-PTHrP, PPx-veh, PPx-PTHrP assessed PTHrP receptor expression, glucose homeostasis. Results PTHrP-receptor, but not ligand, significantly up-regulated islets from PPx compared sham-operated mice. This suggests exogenous could further after PPx. did affect body weight, blood glucose, plasma insulin, insulin sensitivity, sham Glucose tolerance improved versus PPx-veh only early stages treatment. As hypothesized, there a significant increase proliferation at days 7 30; however, normalized by day 90, Enhanced translated marked mass Conclusions enhances through increased Future studies will determine functional setting