Genome Sequence of the Edible Cultivated Mushroom Lentinula edodes (Shiitake) Reveals Insights into Lignocellulose Degradation

• DOI: 10.1371/journal.pone.0160336
• Publication Date: 2016-08-08T17:31:40Z
• Authors: Lianfu Chen; Yuhua Gong; Yingli Cai; Wei Liu; Yan Zhou; Yang Xiao; Zhangyi Xu; Yin Liu; Xiaoyu Lei; Gangzheng Wang; Mengpei Guo; Xiaolong Ma; Yinbing Bian
• Keywords: Lentinula; Edible mushroom

Lentinula edodes, one of the most popular, edible mushroom species with a high content proteins and polysaccharides as well unique aroma, is widely cultivated in many Asian countries, especially China, Japan Korea. As white rot fungus lignocellulose degradation ability, L. edodes has potential for application utilization agriculture straw resources. Here, we report its 41.8-Mb genome, encoding 14,889 predicted genes. Through phylogenetic analysis model fungi, evolutionary divergence time Gymnopus luxurians was estimated to be 39 MYA. The carbohydrate-active enzyme genes were compared those other 25 fungal species, 101 lignocellulolytic enzymes identified similar fungi. Transcriptome showed that expression two cellulases 16 transcription factor up-regulated when mycelia 120 minutes cellulose medium versus glucose medium. Our results will foster better understanding molecular mechanism provide basis partial replacement wood sawdust agricultural wastes cultivation.