Effects of Platelet-Rich Plasma (PRP) on a Model of Renal Ischemia-Reperfusion in Rats

• DOI: 10.1371/journal.pone.0160703
• Publication Date: 2016-08-23T17:34:12Z
• Authors: Oriol Martín-Solé; Joan Rodó; Lluís García-Apar...; Josep Blanch; Victoria Cusí; Asteria Albert
• Keywords: Renal ischemia

Renal ischemia-reperfusion injury is a major cause of acute renal failure, causing cell death, permanent decrease blood flow, organ dysfunction and chronic kidney disease. Platelet-rich plasma (PRP) an autologous product rich in growth factors, therefore able to promote tissue regeneration angiogenesis. This has proven its efficacy multiple studies, but not yet been tested on tissue. The aim this work evaluate whether the application PRP rat kidneys undergoing reduces mid-term damage. A total 30 monorrenal Sprague-Dawley male rats underwent for 45 minutes. During ischemia, (PRP Group, n = 15) or saline solution (SALINE was administered by subcapsular injection. Control were contralateral organs removed immediately before start ischemia remaining kidneys. Survival, body weight, flow Doppler ultrasound, volume, biochemistry histopathology determined all subjects kidneys, as applicable. Correlations between these variables searched for. Group showed significantly worse (p 0.045) more histopathological damage (p<0.0001). found histology, serum levels creatinine urea. Our study provides first evidence that treatment with results deterioration kidney's response injury.