Angiostrongylus cantonensis and A. malaysiensis Broadly Overlap in Thailand, Lao PDR, Cambodia and Myanmar: A Molecular Survey of Larvae in Land Snails

• DOI: 10.1371/journal.pone.0161128
• Publication Date: 2016-08-11T13:52:06Z
• Authors: Rutchanee Rodpai; Pewpan M. Intapan; Tongjit Thanchomnang; Oranuch Sanpool; Lakkhana Sadaow; Sakhone Laymanivong; Win Papa Aung; Issarapong Phosuk; Porntip Laummaunwai; Wanchai Maleewong
• Keywords: Anthelmintic Resistance in Veterinary Parasites; Science; Snails; Electron Transport Complex IV; Agricultural and Biological Sciences; Ecological Interactions of Parasites in Ecosystems; 03 medical and health sciences; 0302 clinical medicine; Helminths; Health Sciences; Animals; Parasitism of Snails and Associated Diseases; Small Animals; Angiostrongylus; Biology; Phylogeny; Strongylida Infections; Ecology; Q; R; Life Sciences; Angiostrongylus cantonensis; Thailand; Veterinary; Eosinophilic Meningitis; RNA, Ribosomal; Larva; Insect Science; FOS: Biological sciences; Environmental Science; Physical Sciences; Medicine; Zoology; Research Article

Angiostrongylus cantonensis is a zoonotic nematode parasite causing human eosinophilic meningitis (or meningoencephalitis) worldwide. A closely related species, malaysiensis, might also be pathogen. Larvae were obtained from land snails in Lao PDR, Cambodia, Myanmar and Thailand. We sequenced two nuclear gene regions (nuclear ribosomal ITS2 SSU rRNA) portion of one mitochondrial (COI) these larvae. A. malaysiensis identified. This the first report molecular identification species Cambodia Myanmar. The regional distributions broadly overlap. Phylogenetic relationships inferred including data deposited public databases. All we have potential value distinguishing between Angiostrongylus. COI exhibited greatest intraspecific variation study region (five haplotypes four malaysiensis) suitable for more detailed phylogeographic studies.