Comparison of Human Neonatal and Adult Blood Leukocyte Subset Composition Phenotypes

• DOI: 10.1371/journal.pone.0162242
• Publication Date: 2016-09-09T14:19:23Z
• Authors: Savit B. Prabhu; Deepak K. Rathore; Deepa Nair; Anita Chaudhary; Saimah Raza; Parna Kanodia; Shailaja Sopory; Anna George; Satyajit Rath; Vineeta Bal; Reva Tripathi; Siddharth Ramji; Aruna Batra; Kailash C. Aggarwal; Harish K. Chellani; Sugandha Arya; Nidhi Agarwal; Umesh Mehta; Uma Chandra Mouli...; Nitya Wadhwa; Shinjini Bhatnagar
• Keywords: Immunophenotyping; Monocyte

The human peripheral leukocyte subset composition depends on genotype variation and pre-natal post-natal environmental influence diversity. We quantified this in adults neonates, compared the median values dispersal ranges of various subsets them. confirmed higher frequencies monocytes regulatory T cells (Tregs), similar neutrophils, lower CD8 cells, NKT B1 B gamma-delta neonatal umbilical cord blood. Unlike previous reports, we found eosinophils CD4:CD8 ratios, iNKT CD4 NK neonates. characterized monocyte dendritic cell (DC) far greater detail than previously reported, using recently described surface markers gating strategies observed that neonates had patrolling myeloid (mDC):plasmacytoid DC (pDC) ratios. Our data contribute to South Asian reference for these parameters. differ between different subsets, suggesting differential determination variation. Further, some were more dispersed influences postnatal sources variation, while show opposite pattern developmental process Together, analyses provide interesting biological possibilities future exploration.