TLR-2 Recognizes Propionibacterium acnes CAMP Factor 1 from Highly Inflammatory Strains

• DOI: 10.1371/journal.pone.0167237
• Publication Date: 2016-11-30T19:25:35Z
• Authors: Coralie Lheure; Philippe Alain Gr...; Guillaume Ollagnier; Philippe Morand; Nathalie Désiré; Sophie Sayon; Stéphane Corvec; Jöel Raingeaud; Anne-Geneviève Ma...; Vincent Calvez; Amir Khammari; Frédéric Batteux; Brigitte Dréno; Nicolas Dupin
• Keywords: Propionibacterium Acnes; Interleukin 8; Lipoteichoic acid

Propionibacterium acnes (P. acnes) is an anaerobic, Gram-positive bacteria encountered in inflammatory acne lesions, particularly the pilosebaceous follicle. P. triggers a strong immune response involving keratinocytes, sebocytes and monocytes, target cells during development. Lipoteicoic acid peptidoglycan induce reaction, but no surface protein interacting with Toll-like receptors has been identified. proteins have extracted by lithium stripping shown to CXCL8 production keratinocytes.Far-western blotting identified two proteins, of 24.5- 27.5-kDa size, specifically recognized TLR2. These were characterized, LC-MS/MS, as CAMP factor 1 devoid its signal peptide sequence, N-terminal sequencing. Purified induces activating gene promoter, triggering synthesis mRNA. Antibodies against TLR2 significantly decreased response. For 27 strains used this study, CAMP1-TLR2 binding intensity was modulated appeared be type IB II strains, which produced large amounts CXCL8, whereas most IA1 IA2 presented little or low levels production. The nucleotide sequence displays major polymorphism, defining distinct genetic groups corresponding 14 amino-acid changes from phylotyped moderate high activity, containing 0, 2 IA1, IA2, presenting no, weak binding.Our findings indicate that may contribute virulence, amplifying inflammation reaction through direct interaction