Enhancement of Adipocyte Browning by Angiotensin II Type 1 Receptor Blockade

• DOI: 10.1371/journal.pone.0167704
• Publication Date: 2016-12-19T18:54:12Z
• Authors: Kana Tsukuda; Masaki Mogi; Jun Iwanami; Harumi Kanno; Hirotomo Nakaoka; Xiao-Li Wang; Hui-Yu Bai; Bao-Shuai Shan; Masayoshi Kukida; Akinori Higaki; Toshifumi Yamauchi; Li-Juan Min; Masatsugu Horiuchi
• Keywords: Male; 0303 health sciences; Science; Adipose Tissue, White; Q; R; Cell Differentiation; Thermogenesis; Receptor, Angiotensin, Type 2; Receptor, Angiotensin, Type 1; 3. Good health; Mitochondrial Proteins; Gene Knockout Techniques; Mice; 03 medical and health sciences; Adipocytes, Brown; Medicine; Animals; Energy Metabolism; Research Article

Browning of white adipose tissue (WAT) has been highlighted as a new possible therapeutic target for obesity, diabetes and lipid metabolic disorders, because WAT browning could increase energy expenditure reduce adiposity. The clusters adipocytes that emerge with have named 'beige' or 'brite' adipocytes. Recent reports indicated the renin-angiotensin system (RAS) plays role in various aspects physiology dysfunction. biological effects angiotensin II, major component RAS, are mediated by two receptor subtypes, II type 1 (AT1R) 2 (AT2R). However, functional roles subtypes not defined. Therefore, we examined whether deletion (AT1aR AT2R) may affect white-to-beige fat conversion vivo. AT1a knockout (AT1aKO) mice exhibited increased appearance multilocular droplets upregulation thermogenic gene expression inguinal (iWAT) compared to wild-type (WT) mice. AT2 receptor-deleted did show miniaturization alteration levels iWAT. An vitro experiment using tissue-derived stem cells showed resulted suppression adipocyte differentiation, reduction genes. These results indicate might some on process blockade AT1 be treatment disorders.