Tumor Infiltration in Enhancing and Non-Enhancing Parts of Glioblastoma: A Correlation with Histopathology

• DOI: 10.1371/journal.pone.0169292
• Publication Date: 2017-01-19T19:26:03Z
• Authors: Oliver Eidel; Sina Burth; Jan-Oliver Neumann; Pascal J. Kieslich; Felix Sahm; Christine Jungk; Philipp Kickinger...; Sebastian Bickelh...; Sibu Mundiyanapurath; Philipp Bäumer; Wolfgang Wick; Heinz-Peter Schle...; Karl Kiening; Andreas Unterberg; Martin Bendszus; Alexander Radbruch
• Keywords: Histopathology; Infiltration (HVAC); Stereotactic biopsy

To correlate histopathologic findings from biopsy specimens with their corresponding location within enhancing areas, non-enhancing areas and necrotic on contrast enhanced T1-weighted MRI scans (cT1).In 37 patients newly diagnosed glioblastoma who underwent stereotactic biopsy, we obtained a correlation of 561 1mm3 position the intraoperative cT1 image at 1.5 Tesla. Biopsy points were categorized as (CE), (NE) or (NEC) tissue samples "viable tumor cells", "blood" "necrotic (with without cellular component)". Cell counting was done semi-automatically.NE had highest content viable cells (89% vs. 60% in CE 30% NEC, respectively). Besides, average cell density for NE (3764 ± 2893 cells/mm2) comparable to (3506 3116 cells/mm2), while NEC lower 2713 3239 cells/mm2. If parts bleeds excluded, biopsies tissue" decreased center (NEC, 5804 3480 (4495 3209 (4130 2817 cells/mm2).The appearance (circular enhancement, central necrosis, peritumoral edema) does not correspond its diffuse histopathological composition. is elevated both parts. Hence, our study suggests that contains considerable amounts infiltrative high cellularity which might be considered resection planning.