Rab14 Act as Oncogene and Induce Proliferation of Gastric Cancer Cells via AKT Signaling Pathway

• DOI: 10.1371/journal.pone.0170620
• Publication Date: 2017-01-20T19:00:57Z
• Authors: Bo Guo; Wenjing Wang; Zhenghao Zhao; Qian Li; Kaiyue Zhou; Lingyu Zhao; Lumin Wang; Juan Yang; Chen Huang
• Keywords: 0303 health sciences; Science; Q; Blotting, Western; Cell Cycle; R; Apoptosis; Oncogenes; Adenocarcinoma; Flow Cytometry; Real-Time Polymerase Chain Reaction; Up-Regulation; 3. Good health; Gene Expression Regulation, Neoplastic; Oncogene Protein v-akt; 03 medical and health sciences; Stomach Neoplasms; rab GTP-Binding Proteins; Cell Line, Tumor; Medicine; Humans; Research Article; Cell Proliferation; Signal Transduction

Rab14 is a member of RAS oncogene family, and its dysfunction has been reported to be involved in various types human cancer. However, expression function were still unclear gastric The aim this study was investigate the mechanism cancer cell lines. Quantitative real-time PCR (qRT-PCR) performed 17 adenocarcinoma tissues 4 lines detect Rab14. 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl-tetrazolium bromide (MTT), colony formation flow cytometry assays employed determine proliferative ability, cycle transition apoptosis vitro BGC-823 or SGC-7901 cells. Western blot pathways regulation. In study, we show that presents significant up-regulated among paired tissue samples When overexpressed cells silenced cells, found could modify growth, apoptosis, which accompanied with an obvious regulation CCND1, CDK2 BAX involving AKT signaling pathway. conclusion, provides new evidence on functions as novel tumor potential therapeutic target