Plasmatic extracellular vesicle microRNAs in malignant pleural mesothelioma and asbestos-exposed subjects suggest a 2-miRNA signature as potential biomarker of disease

• DOI: 10.1371/journal.pone.0176680
• Publication Date: 2017-05-04T14:07:27Z
• Authors: Tommaso Cavalleri; Laura Angelici; Chiara Favero; Laura Dioni; Carolina Mensi; Claudia Bareggi; Alessandro Palleschi; Arianna Rimessi; Dario Consonni; Lorenzo Bordini; Aldo Todaro; Valentina Bollati; Angela Cecilia Pe...
• Keywords: Male; Mesothelioma; 0301 basic medicine; Science; Pleural Neoplasms; Q; R; Asbestos; Middle Aged; 3. Good health; MicroRNAs; 03 medical and health sciences; Biomarkers, Tumor; Medicine; Humans; Female; Research Article; Aged

Malignant Pleural Mesothelioma (MPM) is an aggressive cancer mainly caused by asbestos exposure and refractory to current therapies. Specific diagnostic markers for early MPM diagnosis are needed. Changes in miRNA expression have been implicated several diseases cancers, including MPM. We examined if a specific signature plasmatic extracellular vesicles (EV) may help discriminate between malignant pleural mesothelioma patients subjects with Past Asbestos Exposure (PAE).We investigated 23 19 cancer-free past (PAE). screened 754 miRNAs EVs OpenArray found 55 differential using logistic regression models adjusted age, sex, BMI, smoking. Among the top-20 chosen validation Real time PCR, 16 were confirmed. Using receiver operating characteristic (ROC) curve analysis, most discriminating combination was given miR-103a-3p + miR-30e-3p, which generated AUC of 0.942 (95% CI 0.87-1.00), sensitivity 95.5% specificity 80.0%. multivariate Cox analysis gender, BMI smoking we Hazard Ratio above median 0.37 (95%CI 0.13-1.13) 0.51 0.17-1.52) miR-30e-3p.This study suggests EV-associated miR-30e-3p able from PAE subjects. Larger prospective studies needed confirm these two-miRNA alone or other biomarkers as tools