4'-Hydroxywogonin suppresses lipopolysaccharide-induced inflammatory responses in RAW 264.7 macrophages and acute lung injury mice

• DOI: 10.1371/journal.pone.0181191
• Publication Date: 2017-08-09T15:06:04Z
• Authors: Chao Fan; Le-Hao Wu; Gu-Fang Zhang; Fangfang Xu; Shuo Zhang; Xiuli Zhang; Lei Sun; Yang Yu; Yan Zhang; Richard D. Ye
• Keywords: Proinflammatory cytokine; IκBα; IκB kinase

4'-Hydroxywogonin (4'-HW), a flavonoid, has been isolated from various plants and shown to inhibit NO production in macrophages. However, the molecular mechanisms its vivo activity have not determined. Our study aimed investigate underlying anti-inflammatory effects of 4'-HW vitro vivo. We showed that potently reduced expression levels COX-2 iNOS as well their products, prostaglandin E2 (PGE2) nitric oxide (NO) respectively, LPS-stimulated RAW 264.7 also suppressed LPS-induced pro-inflammatory cytokines at mRNA protein levels. Moreover, blocked interaction TAK1 TAB1 macrophages, resulting an inhibition TAK1/IKK/NF-κB signaling pathway. Furthermore, phosphorylation MAPKs PI3/Akt pathways was significantly decreased intracellular reactive oxygen species (ROS) level. The effect confirmed exhibited potent protective against ALI mice. These findings indicate suppresses response It is likely TAK1/IKK/NF-κB, PI3/AKT contribute 4'-HW. suggests may be important functional constituent potential value developed novel agent.