Detection of anti-HspX antibodies and HspX protein in patient sera for the identification of recent latent infection by Mycobacterium tuberculosis

• DOI: 10.1371/journal.pone.0181714
• Publication Date: 2017-08-16T19:15:19Z
• Authors: Jorge Castro-Garza; Paola García-Jacobo; Lydia G. Rivera-M...; Frederick D. Quinn; James Barber; Russell Karls; Debra Haas; Shelly Helms; Tuhina Gupta; Henry Blumberg; Jane Tapia; Itza Luna-Cruz; Adrián Rendon; Javier Vargas-Vil...; Lucio Vera-Cabrera; Cristina Rodrígue...
• Keywords: Male; 0301 basic medicine; 570; Science; Immunology; 610; Enzyme-Linked Immunosorbent Assay; Cross Reactions; FOS: Health sciences; Microbiology; 03 medical and health sciences; Bacterial Proteins; Latent Tuberculosis; Virology; Health Sciences; Pathology; Humans; Tuberculosis; alpha-Crystallins; Biology; Antibody; Immunology and Microbiology; Antigens, Bacterial; Latent tuberculosis; FOS: Clinical medicine; Q; R; Gastrointestinal Viral Infections and Vaccines Development; Life Sciences; Mycobacterium tuberculosis; Leptospirosis: Zoonotic Disease and Global Impact; 3. Good health; Infectious Diseases; Immunoglobulin M; Immunoglobulin G; FOS: Biological sciences; Medicine; QR Microbiología; Female; Parasitology; Research Article

Mycobacterium tuberculosis is a pathogen causing (TB) spectrum of disease including acute and asymptomatic latent stages. Identifying treating latently-infected patients constitutes one the most important impediments to TB control efforts. Those individuals can remain undiagnosed for decades serving as potential reservoirs reactivation. Tests accurate diagnosis infection currently are unavailable. HspX protein (α-crystallin), encoded by Rv2031c gene, produced in vitro M. during stationary growth phase hypoxic or acidic culture conditions. In this study, using standard, Luminex xMAP® bead capture ELISA, respectively, we report on detection anti-HspX IgG IgM antibodies sera from patients. For antibody screen, levels were similar between non-infected active patients; however, classified into group with showed higher values (p = 0.003) compared Using antigen assay, was detected 56.5% putative cases (p< 0.050) background median an average 9,900 pg/ml range 1,000 36,000 pg/ml. Thus, presence may be markers TB.