Gestational diabetes mellitus is associated with increased pro-migratory activation of vascular endothelial growth factor receptor 2 and reduced expression of vascular endothelial growth factor receptor 1

• DOI: 10.1371/journal.pone.0182509
• Publication Date: 2017-08-17T14:10:41Z
• Authors: Felipe Troncoso; Jesenia Acurio; Kurt Herlitz; Claudio Aguayo; Patricio Bertoglia; Enrique Guzman-Gu...; Marco Loyola; Marcelo Gonzalez; Meriem Rezgaoui; Gernot Desoye; Carlos Escudero
• Keywords: CD31

Placentas from gestational diabetes mellitus (GDM) are often hypervascularized; however, participation of vascular endothelial growth factor (VEGF) and its receptors in this placental adaptation is unclear. We aimed to test whether changes phosphorylation tyrosine 951 or 1175 (pY951 pY1175) the receptor 2 (KDR) associated with proangiogenic state observed placentas GDM. obtained samples women normal pregnancies (n = 24) GDM 18). measured relative expression markers for cell number (CD31, CD34), VEGF, 1 (Flt-1), KDR, pY951 pY1175 KDR homogenate. Immunohistochemistry blood vessels were performed using CD34. Proliferation migration human umbilical vein cells (HUVEC) pregnancy determined absence presence conditioned medium (CM) harvested normoglycemic HUVEC cultures. was more CD31 CD34 protein compared pregnancy. High number, but reduced area found Reduced Flt-1 levels (mRNA protein) mRNA, higher No significant Y951-or Y1175-phosphorylation found. did not alter proliferation HUVECs, enhanced migration. Conditioned cultures amount, tube formation capacity isolated pregnancies. The data indicate that high pro-migratory activation reflecting a