Metabolomics reveals that vine tea (Ampelopsis grossedentata) prevents high-fat-diet-induced metabolism disorder by improving glucose homeostasis in rats

• DOI: 10.1371/journal.pone.0182830
• Publication Date: 2017-08-16T15:12:55Z
• Authors: Wenting Wan; Baoping Jiang; Le Sun; Lijia Xu; Peigen Xiao
• Keywords: Carbohydrate Metabolism; Gluconeogenesis; Homeostasis

Vine tea (VT), derived from Ampelopsis grossedentata (Hand.-Mazz.) W.T. Wang, is an alternative that has been consumed widely in south China for hundreds of years. It shown drinking VT on a daily basis improves hyperlipidemia and hyperglycemia. However, little known about the preventive functions metabolic dysregulation potential pathological mechanisms involved. This paper elucidates effects lipid glucose metabolism using rats maintained high-fat-diet (HFD) attempt to explain involved.Sprague Dawley (SD) were divided into five groups: group given normal rat chow water (control group); HFD (HFD Pioglitazone (PIO group), 5 mg /kg; groups one two doses VT: 500 mg/L or 2000 mg/L. After 8 weeks, changes food intake, consumption, body weight, serum hepatic biochemical parameters determined. Moreover, liver samples isolated pathology histology liquid chromatography-mass spectrometry (LC-MS)-based metabolomic research.VT reduced levels total cholesterol, decreased area under curve insulin tolerance test visibly impaired accumulation. Metabolomics showed treatment modulated contents intermediates linked (including gluconeogenesis glycolysis), TCA cycle, purine amino acid metabolism.The current results demonstrate may prevent impairments induced by consumption HFD. These be caused improved energy-related gluconeogenesis, glycolysis cycle), metabolism, HFD-fed rats.