Human platelet interaction with E. coli O111 promotes tissue-factor-dependent procoagulant activity, involving Toll like receptor 4

• DOI: 10.1371/journal.pone.0185431
• Publication Date: 2017-09-28T18:02:44Z
• Authors: Valeria Matus; J. Guillermo Vale...; Patricia Hidalgo; L. María Pozo; Olga Panes; Aniela Wozniak; Diego Mezzano; Jaime Pereira; Claudia G. Sáez
• Keywords: Protease-activated receptor

Platelets have a major role in clotting activation and contribute to the innate immune response during systemic infections. Human platelets contain tissue factor (TF) express functional Toll-like receptor 4 (TLR4). However, of TLR4 triggering procoagulant properties platelets, upon challenge with bacteria, is yet unknown. Our hypothesis that E. coli O111-TLR4 interaction activates elicits their activity. We demonstrated strain, but not ultrapure LPS, increased surface P-selectin expression, platelet dependent TF activity (TF-PCA) prompted faster thrombin generation (TG). Blockade resulted decreased activation, TF-PCA TG, revealing participation this on platelets. results provide novel mechanism by which individuals bacterial infections would an incidence blood clots. Furthermore, identification as regulators effect O111 might represent therapeutic target reduce devastating consequences hemostatic disorder sepsis.