Transcription analysis of the responses of porcine heart to Erysipelothrix rhusiopathiae

• DOI: 10.1371/journal.pone.0185548
• Publication Date: 2017-10-04T17:40:14Z
• Authors: Chao Kang; Qiang Zhang; Weifeng Zhu; Chengzhi Cai; Xiaomei Sun; Meilin Jin
• Keywords: Erysipelothrix rhusiopathiae

Erysipelothrix rhusiopathiae (E. rhusiopathiae) is the causative agent of swine erysipelas. This microbe has caused great economic losses in China and other countries. In this study, high-throughput cDNA microarray assays were employed to evaluate host responses porcine heart E. gain additional insights into its pathogenesis. A total 394 DE transcripts detected active virulent infection group compared with PBS at 4 days post-infection. Moreover, 262 upregulated 132 downregulated. Differentially expressed genes involved many vital functional classes, including inflammatory immune responses, signal transduction, apoptosis, transport, protein phosphorylation dephosphorylation, metabolic processes, chemotaxis, cell adhesion, innate responses. Pathway analysis demonstrated that most significant pathways Chemokine signaling pathway, NF-kappa B TLR CAMs, systemic lupus erythematosus, chemokine Cytokine–cytokine receptor interaction, PI3K-Akt Phagosome, HTLV-I infection, Measles, Rheumatoid arthritis natural-killer-cell-mediated cytotoxicity. The reliability our data was verified by performing quantitative real-time PCR. study first document response piglet infection. observed gene expression profile could help screen potential agents can reduce prevalence rhusiopathiae. might also provide underlying pathological changes occur pigs infected