Co-delivery of micronized urinary bladder matrix damps regenerative capacity of minced muscle grafts in the treatment of volumetric muscle loss injuries

• DOI: 10.1371/journal.pone.0186593
• Publication Date: 2017-10-17T17:44:45Z
• Authors: Stephen M. Goldman; Benjamin T. Corona
• Keywords: Muscle tissue; Matrix (chemical analysis)

Minced muscle grafts (MG) promote de novo fiber regeneration and neuromuscular strength recovery in small large animal models of volumetric loss. The most noteworthy limitation this approach is its reliance on a finite supply donor tissue. To address shortcoming, study sought to evaluate micronized acellular urinary bladder matrix (UBM) as scaffolding vivo expansion MG therapy rat model. Rats received loss injuries the tibialis anterior their left hind limb which were either untreated or repaired with minced graft at dosages 50% 100% defect mass, isolation, an product consisting combination two putative therapies delivered dosage mass. survived 2 8 weeks post injury before functional (in strength), histological, morphological, biochemical analyses performed. treated exhibited improved function relative VML after week time period following injury. This improvement capacity, however, was accompanied concomitant reduction mediated regeneration, evidenced cell lineage tracing enable by transgenic GFP expressing donor, mixed histological outcome indicating coincident fibrous deposition interspersed islands nascent fibers. Furthermore, quantitative immunofluorescence transcriptional analysis point suggests exacerbated immune response UBM possible nidus for observed suboptimal regenerative outcome. Moving forward, efforts related development should carefully consider effects host candidate biomaterials order avoid undesirable dysregulation pro-regenerative cross talk between system myogenic processes.