ChrASO, the chromate efflux pump of Shewanella oneidensis, improves chromate survival and reduction

• DOI: 10.1371/journal.pone.0188516
• Publication Date: 2017-11-22T18:27:24Z
• Authors: Hiba Baaziz; Cyril Gambari; Anne Boyeldieu; Amine Ali Chaouche; Radia Alatou; Vincent Méjean; Cécile Jourlin-Ca...; Michel Fons
• Keywords: Shewanella oneidensis; Chromate conversion coating; Efflux; Potassium chromate

The chromate efflux pump encoding gene chrASO was identified on the chromosome of Shewanella oneidensis MR1. Although is expressed without chromate, its expression level increases when Cr(VI) added. When deleted, resulting mutant ΔchrASO exhibits a sensitive phenotype compared to that wild-type strain. Interestingly, heterologous in E. coli confers resistance high concentration. Moreover, S. and significantly improves reduction. This effect could result either from extracytoplasmic reduction or better cell survival leading enhanced