Bicarbonate buffered peritoneal dialysis fluid upregulates angiopoietin-1 and promotes vessel maturation

• DOI: 10.1371/journal.pone.0189903
• Publication Date: 2017-12-18T18:37:23Z
• Authors: Gwendolyn Eich; Maria Bartosova; Christian Tischer; Tanja Tamara Wlod...; Betti Schaefer; Sebastian Pichl; Nicole Kraewer; Bruno Ranchin; Karel Vondrak; Max Christoph Liebau; Thilo Hackert; Claus Peter Schmitt
• Keywords: Angiopoietin; Peritoneal fluid; Bicarbonate

Ultrafiltration decline is a progressive issue for patients on chronic peritoneal dialysis (PD) and can be caused by angiogenesis induced PD fluids. A recent pediatric trial suggests better preservation of ultrafiltration with bicarbonate versus lactate buffered fluid; underlying molecular mechanisms are unknown.Angiogenic cytokine profile, tube formation capacity Receptor Tyrosine Kinase translocation were assessed in primary human umbilical vein endothelial cells following incubation (BPDF) (LPDF), pH neutral fluid low glucose degradation product content buffered, acidic high (CPDF). Peritoneal biopsies from age-, PD-vintage- dialytic exposure matched, peritonitis-free children underwent automated histomorphometry immunohistochemistry.In angiopoietin-1 mRNA protein abundance increased 200% upon BPDF, but decreased 70% LPDF as compared to medium control; angiopoietin-2 remained unchanged. Angiopoietin-1/Angiopoietin-2 ratio was 15 3-fold BPDF medium. Time-lapse microscopy network analysis demonstrated less cell CPDF incubation. translocated the membrane not or incubated cells. In dialyzed vessels larger CD31 positive endothelium higher treated LPDF.Bicarbonate promotes vessel maturation via upregulation vitro dialysis. Our findings suggest mechanism observed superior content.