MiR-34a regulates the invasive capacity of canine osteosarcoma cell lines

• DOI: 10.1371/journal.pone.0190086
• Publication Date: 2018-01-02T19:55:39Z
• Authors: Cecilia M. Lopez; Peter Y. Yu; Xiaoli Zhang; Ayse Selen Yilmaz; Cheryl A. London; Joelle M. Fenger
• Keywords: KLF4

Background Osteosarcoma (OSA) is the most common bone tumor in children and dogs; however, no substantial improvement clinical outcome has occurred either species over past 30 years. MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression play a fundamental role cancer. The purpose of this study was to investigate potential contribution miR-34a loss biology canine OSA, well-established spontaneous model human disease. Methodology principal findings RT-qPCR demonstrated levels were significantly reduced primary OSA tumors cell lines as compared normal osteoblasts. In stably transduced with empty vector or pre-miR-34a lentiviral constructs, overexpression inhibited cellular invasion migration but had effect on proliferation cycle distribution. Transcriptional profiling OSA8 cells possessing enforced dysregulation numerous genes, including significant down-regulation multiple putative targets miR-34a. Moreover, ontology analysis down-regulated target genes showed enrichment several biological processes related motility. Lastly, we validated changes expression, decreased KLF4, SEM3A, VEGFA transcripts overexpressing identified KLF4 direct Concordant these data, tissues increased genes. Conclusions These data demonstrate contributes suggest may promote pattern contributing metastatic phenotype OSA.