High fat diet sensitizes fibromyalgia-like pain behaviors in mice via tumor necrosis factor alpha

• DOI: 10.1371/journal.pone.0190861
• Publication Date: 2018-02-14T13:29:10Z
• Authors: Dan Tian; Miao Tian; Leilei Zhang; Peng Zhao; Yunfeng Cui; Jinlong Li
• Keywords: Dorsal root ganglion

Fibromyalgia (FM) and obesity are closely related. However, little is known about how contributes to FM. Importantly, adequate evidence has shown that tumor necrosis factor alpha (TNF-α) plays a critical role in obesity. Thus, we hypothesized obesity-induced TNF-α release may potentiate FM-associated pain. To test this hypothesis, investigated the of development FM-like pain mouse model acid saline injection-induced Consistent with previous reports, showed repeated injections induced bilateral mechanical hyperalgesia, effect lasted for at least 4 days after injections. This phenomenon was associated increased levels plasma, muscles, spinal cord. Furthermore, found 24 weeks high fat diet treatment significantly potentiated saline-induced hyperalgesia. High diet-treated mice exhibited robustly cord compared low mice. Additionally, using immunofluorescence staining, number positive cells dorsal root ganglion (DRG) injections, further sensitized increase. Finally, reported behaviors were abolished TNFRp55-/- mice, confirming Taken together, our results suggested sensitize via increasing DRG.