Lipidomic analysis of immune activation in equine leptospirosis and Leptospira-vaccinated horses

• DOI: 10.1371/journal.pone.0193424
• Publication Date: 2018-02-23T19:20:06Z
• Authors: Paul L. Wood; Margaret Steinman; Erdal Erol; Craig Carter; Undine Christmann; Ashutosh Verma
• Keywords: Lipidome

Currently available diagnostic assays for leptospirosis cannot differentiate vaccine from infection serum antibody. Several leptospiral proteins that are upregulated during have been described, but their utility as a marker is still unclear. In this study, we undertook lipidomics approach to determine if there any differences in the lipid profiles of horses naturally infected with pathogenic Leptospira spp. and vaccinated against commercially bacterin. Utilizing high-resolution mass spectrometry analytical platform, demonstrate cyclic phosphatidic acids, diacylglycerols, hydroperoxide oxidation products choline plasmalogens elevated well horses. Other lipids interest were triacylglycerols only sphingomyelins increased This first report looking at equine lipidome vaccination.